LOCUS       GLYCLEAV-PWY 6863 bp     DNA  linear    SYN       30-JUL-2026
DEFINITION  Synthetic construct GLYCLEAV-PWY_T3; designed by SynPAL design pipeline.
ACCESSION   GLYCLEAV-PWY_T3
VERSION     GLYCLEAV-PWY_T3
KEYWORDS    SynPAL; synthetic construct.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /note="designed by SynPAL design pipeline"
     misc_feature    1..387
                     /note="flanking_up: upstream regulatory flank [40,800]bp"
                     /note="source organism: Sinorhizobium meliloti 1021"
     promoter        1..387
                     /note="placeholder promoter (native upstream flank)"
     gene            388..1527
                     /gene="gcvT"
                     /locus_tag="gcvT"
     CDS             388..1527
                     /gene="gcvT"
                     /locus_tag="gcvT"
                     /organism="Sinorhizobium meliloti 1021"
                     /note="native genomic strand -"
     gene            1528..1890
                     /gene="gcvH"
                     /locus_tag="gcvH"
     CDS             1528..1890
                     /gene="gcvH"
                     /locus_tag="gcvH"
                     /organism="Sinorhizobium meliloti 1021"
                     /note="native genomic strand -"
     gene            1891..4755
                     /gene="gcvP"
                     /locus_tag="gcvP"
     CDS             1891..4755
                     /gene="gcvP"
                     /locus_tag="gcvP"
                     /organism="Sinorhizobium meliloti 1021"
                     /note="native genomic strand -"
     misc_feature    4756..5042
                     /note="flanking_down: downstream regulatory flank [40,800]bp"
                     /note="source organism: Sinorhizobium meliloti 1021"
     terminator      4756..5042
                     /note="placeholder terminator (native downstream flank)"
     misc_feature    5043..5367
                     /note="flanking_up: upstream regulatory flank [40,800]bp"
                     /note="source organism: Escherichia coli K-12 substr. MG1655"
     promoter        5043..5367
                     /note="placeholder promoter (native upstream flank)"
     gene            5368..6792
                     /gene="lpd"
                     /locus_tag="lpd"
     CDS             5368..6792
                     /gene="lpd"
                     /locus_tag="lpd"
                     /organism="Escherichia coli K-12 substr. MG1655"
                     /note="native genomic strand +"
     misc_feature    6793..6863
                     /note="flanking_down: downstream regulatory flank [40,800]bp"
                     /note="source organism: Escherichia coli K-12 substr. MG1655"
     terminator      6793..6863
                     /note="placeholder terminator (native downstream flank)"
ORIGIN
        1 tggccacctc tcttcggtct tccctaaccg ctgcccaaag aaattgcaaa ggccgatttt
       61 gccgcacttg tcgcggagga tgaaatcagg gcgacaaaaa tatgtttggt cgcgacatag
      121 gcccttgcca aaatccggcc ttcgccgtta tctggattcg acctcgttgg gagaaaccgg
      181 ttcgatccgg tgccgaagga gcaaccgccc cggaaactct caggccaaag gaccagcaag
      241 gtgccggtag gactctggag agaagcgttc gcgctcgccg aagggataac aatctcaggc
      301 aaagggacag agggggctcg aatttgtcgg cgcatgacgc cggtattgtg agccggcgcg
      361 aaaacgcgcc agacctggag gccggacttg gaagacgttt ccaccttgaa acacacgcct
      421 ttgcacgcgc tgcacctttc gctcggtgca cgcatggtgc ccttcgccgg ttacgacatg
      481 ccggtccaat acccggaagg cgtattgaag gagcacctgc atacccgcgc cgccgccggc
      541 ctcttcgacg tgtcccacat gggacagatc gtcgtgcggc cgaaatcggg aaaaatcgag
      601 gacgcggcgc tggcgctgga aaagctcgtg ccggcggatg ttctcggcct cgcggaaggc
      661 cgacagcgct acggcctctt caccaatgcc gcaggcggca ttctcgacga cctgatgatc
      721 gtcaatcgcg gcgatcatct cttcctcgtc gtcaacgcgg cctgcaagga tgccgatctt
      781 gcgcatctca aggacggtct cggcagcgtc tgcgacgtga ccatgctgac cgaccgtgcc
      841 ctgatcgccc ttcagggacc ccgggccgga gcggtcctct gcgaactctg ggcggacgtc
      901 tcgtcgatgc gcttcatgga tgtcaccgaa gccgaccttc acgatgtgag ctgcatcatc
      961 tcccgctccg gctacaccgg cgaggatggc ttcgagatat ccattcccgc ggaagcggct
     1021 gtggatgtga cgcagcgcct gctcgaacat cccgacgttc tgcccatcgg gctcggcgcg
     1081 cgcgactcgc tccgcctcga agcggggctt tgtctctatg gcaacgatat cgacacgaat
     1141 acgagcccca tcgaagcagg cctcgaatgg gcgatccaga aaagccgccg cgccggcggt
     1201 gagcgcgccg gcggctttcc gggcgccggc cggatactgg ccgagctaac cgacggagtt
     1261 tccaggcggc gtgtcggcct gaggcccgaa ggccgggcgc cggtccgcgg caatgccaat
     1321 ctcttcgccg atgaggaggg cagaacggcg gcaggaaccg tcacctccgg cggcttcggt
     1381 ccaagcgtgg atggaccggt cgccatgggc tatgtcgacg ccgagcacgc cgaagtcggc
     1441 accagactgt tcgcagaggt gcgcggcaaa tacctgccca tcgccgtcac tgccctgccc
     1501 ttcatcaaac aaacctacaa acgctgaatg ctgaaattca ccgaggaaca cgaatggctg
     1561 aagatcgatg gcgacgtcgc gaccgtcggc atcacggagc atgccgctgg ccagctcggc
     1621 gatcttgtct tcgtggaact gccggaagcg ggtgccagct tctccaaggg cgactcggcc
     1681 gcgacggtcg agtccgtcaa ggcggcttcg gacgtctatt gtccgcttga tggagaaatc
     1741 gtcgaggtca atcaggcgat cgtcgacgat ccgtcgctcg tcaacagcga tccgcaaggc
     1801 gcggcctggt tcttcaagct gaagctcgcc gatccggacg ctgccaatgc gctcctggac
     1861 gaagcggcct acaaggagtt cgtcgcctga atgagcatgc ccaaggactt taccttcacc
     1921 gattacaagc cttacgactt cgccaatcga cgtcacatcg gcccttcgcc ggccgagatg
     1981 gacgagatgc tgaaggtcgt cggctatccg agcctcgacg cgctcataga cgacaccgtg
     2041 ccgccgtcga tccgccagcg gacgccgctt gcctggggcg cgccgatgac cgagcgcgag
     2101 gcactcgaca agctgcgcga gacggcgaac cgcaaccgga agctcgtctc cctgatcggt
     2161 cagggctact acgggaccat caccccgccg gtcatccagc gcaatatcct cgagaatccg
     2221 gcctggtaca ccgcctatac gccctatcag ccggaaatca gccaggggcg gctggaagcc
     2281 ctgctcaact accagaccat ggtttgcgat ttgaccggcc tggacgtcgc gaacgcctcg
     2341 ttgctcgacg aggcgacggc ggcagccgaa gcaatggcca ttgccgagcg cgtcgcaaag
     2401 tccaaggcga aggcgttttt catcgatgag aactgccatc cccagacgat tgcgctcctg
     2461 aagacccggg cggagccgct cggctggcag atcgtcctcg gcgatccgtt cgaggatctg
     2521 gacgctgccg gcgtcttcgg cgcgattttc cagtaccccg gtacctacgg ccatgtccgc
     2581 gacttctcgg gcctgatcgc caaactccat gggcaggggg caatcgccgc cgtagcagcc
     2641 gatccgctgg cgctggcgct tctgaagtct ccgggtgaaa tgggcgccga catcgcgatc
     2701 ggttcgaccc agcgcttcgg cgttccggtc ggctacggcg gaccgcacgc cgcctatatg
     2761 gcggtcaagg atgcctataa gcgctccatg cccggccgcc tcgtgggcgt gtcggtcgat
     2821 gcgcggggca accgcgccta ccggctgtcg ctccagaccc gtgaacagca tatccggcgc
     2881 gaaaaggcga catcgaacat ctgtaccgcg caggtgctgc ttgccgtcat ggcctccatg
     2941 tatgcggtct tccacggtcc ggacggaata aaggcgatcg cccagagcgt gcaccagaag
     3001 acggtccggc ttgccatggg attggagaag ctcggctata ccgtcgagcc ggatgtcttc
     3061 ttcgatacga tcaccgtcga agtcggcaag ctgcaaggca tcatcctcaa agccgccgtg
     3121 gccgaggagg taaaccttcg caagatcggc acgaccagga tcggcatcag tctcgacgag
     3181 cgttcgaggc cggtcacgct cgaggccgtc tggcgcgcct tcgggggcga tttcaaggtc
     3241 gaggagttcg agccggacta ccgcctgccg caggaactgc tgcgcaccag cgcctatctc
     3301 acccatccga tcttccatat gaaccgcgcc gagagcgaga tgacgcgtta catgcgccgc
     3361 ctcgccgatc gcgatcttgc gctcgaccgc gcgatgatcc cgctcggctc ctgcacgatg
     3421 aagctcaacg cgaccgcgga gatgctgccg atcacctggc cggagttctc ggaaatccat
     3481 cccttcgtgc cggccgacca ggcactcggc tatcagcatc tcatcgaaga cctgtcgcaa
     3541 aagctctgcg cgatcaccgg ctacgatgcg atctcgatgc agccgaactc gggtgcccag
     3601 ggtgaatatg ccggtctgct cgcgatccgc gcctaccata tcgccaacgg caacgagcat
     3661 cgcgacgtct gcctgatacc gacctcggcg cacggcacca acccggcctc ggcgcagatg
     3721 gccggcatga aggtggtggt cgtcaaggtg agcgacgccg gcgagatcga tatggacgat
     3781 ttccgcgcca aagcggagca gtatgcggac accctctcct gctgcatgat cacctatccc
     3841 tcgacgcacg gcgtgttcga ggagaacgtc cgcgaagtct gcgaggtcgt gcataagcac
     3901 ggtggccagg tctatctgga cggcgccaac atgaatgcga tggttggcct ttcccgccct
     3961 ggcgacatcg gctcggatgt cagccatttg aacctgcaca agaccttctg cattccgcat
     4021 ggcggcggcg gtcccggcat gggcccgatc ggcgtcaagt cgcatctcgc ccccttcctt
     4081 ccgggacacc cgcagaccga cgggcacgag ggcgccgtat ccgccgcacc gttcgggtcg
     4141 gcctcgatcc tgccgatctc ctggagctac tgcctgatga tgggcggcga aggcctgacg
     4201 caagcgacca aggtggcgat cctcaacgcc aattacgtcg ccgcccggct gaagggtgcc
     4261 tatgacgtgc tctacaagtc ggcgaaaggc cgggtcgcgc atgaatgcat catcgatacc
     4321 cgtccgctcg ccgaaagcgc cggcgtcacg gtcgatgacg tcgccaagcg cctgatcgac
     4381 tgcggcttcc atgcaccgac gatgagctgg ccggtcgcgg gcacactgat gatcgaaccc
     4441 acggaatcgg aaacgaaggc cgagctcgac cgcttctgcg acgccatgct ggcaatccgc
     4501 gaagaggccc gcgccatcga ggacggccgg atggacaagg tcaacaatcc gctgaagaac
     4561 gcgccgcata cggtcgagga cctcgtcggc gactgggacc ggccctattc gcgcgagcag
     4621 gcctgcttcc caccgggcgc cttccgcgtg gacaaatact ggtcgccggt caaccgtgtc
     4681 gacaatgtct atggcgaccg caatctcgtc tgcacctgcc cgccgatcga gagctacgcg
     4741 gaagcggcgg aatgatgacg tgatccgatc gggcgcggtt caagcgccgc gcctgcctgc
     4801 cacgtcctga aagcaaaaag cccggctcct aagccgggct tggcctactg catgtctcct
     4861 ttaatcgtat ccgattaaag gacaaaaaca tgcagcagat caaagtgctg cagcgacctt
     4921 tgcgcgtctg ataagacgcg cggcgctgta agcacttccc ggagaagtgc ggagcagctt
     4981 tccctctgca gaaaagaaaa agccgcccgg aggcggcctc ttcgtgtcct gagagatgcg
     5041 gtagtaaaag agccggccca acggccggct tttttctggt aatctcatga atgtattgag
     5101 gttattagcg aatagacaaa tcggttgccg tttgttgttt aaaaattgtt aacaattttg
     5161 taaaataccg acggatagaa cgacccggtg gtggttaggg tattacttca cataccctat
     5221 ggatttctgg gtgcagcaag gtagcaagcg ccagaatccc caggagctta cataagtaag
     5281 tgactggggt gagggcgtga agctaacgcc gctgcggcct gaaagacgac gggtatgacc
     5341 gccggagata aatatataga ggtcatgatg agtactgaaa tcaaaactca ggtcgtggta
     5401 cttggggcag gccccgcagg ttactccgct gccttccgtt gcgctgattt aggtctggaa
     5461 accgtaatcg tagaacgtta caacaccctt ggcggtgttt gcctgaacgt cggctgtatc
     5521 ccttctaaag cactgctgca cgtagcaaaa gttatcgaag aagccaaagc gctggctgaa
     5581 cacggtatcg tcttcggcga accgaaaacc gatatcgaca agattcgtac ctggaaagag
     5641 aaagtgatca atcagctgac cggtggtctg gctggtatgg cgaaaggccg caaagtcaaa
     5701 gtggtcaacg gtctgggtaa attcaccggg gctaacaccc tggaagttga aggtgagaac
     5761 ggcaaaaccg tgatcaactt cgacaacgcg atcattgcag cgggttctcg cccgatccaa
     5821 ctgccgttta ttccgcatga agatccgcgt atctgggact ccactgacgc gctggaactg
     5881 aaagaagtac cagaacgcct gctggtaatg ggtggcggta tcatcggtct ggaaatgggc
     5941 accgtttacc acgcgctggg ttcacagatt gacgtggttg aaatgttcga ccaggttatc
     6001 ccggcagctg acaaagacat cgttaaagtc ttcaccaagc gtatcagcaa gaaattcaac
     6061 ctgatgctgg aaaccaaagt taccgccgtt gaagcgaaag aagacggcat ttatgtgacg
     6121 atggaaggca aaaaagcacc cgctgaaccg cagcgttacg acgccgtgct ggtagcgatt
     6181 ggtcgtgtgc cgaacggtaa aaacctcgac gcaggcaaag caggcgtgga agttgacgac
     6241 cgtggtttca tccgcgttga caaacagctg cgtaccaacg taccgcacat ctttgctatc
     6301 ggcgatatcg tcggtcaacc gatgctggca cacaaaggtg ttcacgaagg tcacgttgcc
     6361 gctgaagtta tcgccggtaa gaaacactac ttcgatccga aagttatccc gtccatcgcc
     6421 tataccgaac cagaagttgc atgggtgggt ctgactgaga aagaagcgaa agagaaaggc
     6481 atcagctatg aaaccgccac cttcccgtgg gctgcttctg gtcgtgctat cgcttccgac
     6541 tgcgcagacg gtatgaccaa gctgattttc gacaaagaat ctcaccgtgt gatcggtggt
     6601 gcgattgtcg gtactaacgg cggcgagctg ctgggtgaaa tcggcctggc aatcgaaatg
     6661 ggttgtgatg ctgaagacat cgcactgacc atccacgcgc acccgactct gcacgagtct
     6721 gtgggcctgg cggcagaagt gttcgaaggt agcattaccg acctgccgaa cccgaaagcg
     6781 aagaagaagt aatttttcgt ttgccggaac atccggcaat taaaaaagcg gctaaccacg
     6841 ccgctttttt tacgtctgca att
//
