LOCUS       PWY-5046_T1  4779 bp     DNA  linear    SYN       30-JUL-2026
DEFINITION  Synthetic construct PWY-5046_T1; designed by SynPAL design pipeline.
ACCESSION   PWY-5046_T1
VERSION     PWY-5046_T1
KEYWORDS    SynPAL; synthetic construct.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /note="designed by SynPAL design pipeline"
     misc_feature    1..40
                     /note="flanking_up: upstream regulatory flank [40,800]bp"
                     /note="source organism: Bacillus subtilis subtilis 168"
     promoter        1..40
                     /note="placeholder promoter (native upstream flank)"
     gene            41..1465
                     /gene="lpdV"
                     /locus_tag="lpdV"
     CDS             41..1465
                     /gene="lpdV"
                     /locus_tag="lpdV"
                     /organism="Bacillus subtilis subtilis 168"
                     /note="native genomic strand -"
     gene            1466..2458
                     /gene="bkdAA"
                     /locus_tag="bkdAA"
     CDS             1466..2458
                     /gene="bkdAA"
                     /locus_tag="bkdAA"
                     /organism="Bacillus subtilis subtilis 168"
                     /note="native genomic strand -"
     gene            2459..3442
                     /gene="bkdAB"
                     /locus_tag="bkdAB"
     CDS             2459..3442
                     /gene="bkdAB"
                     /locus_tag="bkdAB"
                     /organism="Bacillus subtilis subtilis 168"
                     /note="native genomic strand -"
     gene            3443..4717
                     /gene="bkdB"
                     /locus_tag="bkdB"
     CDS             3443..4717
                     /gene="bkdB"
                     /locus_tag="bkdB"
                     /organism="Bacillus subtilis subtilis 168"
                     /note="native genomic strand -"
     misc_feature    4718..4779
                     /note="flanking_down: downstream regulatory flank [40,800]bp"
                     /note="source organism: Bacillus subtilis subtilis 168"
     terminator      4718..4779
                     /note="placeholder terminator (native downstream flank)"
ORIGIN
        1 aacaatatcg tattgaatag acagacagga gtgagtcacc atggcaactg agtatgacgt
       61 agtcattctg ggcggcggta ccggcggtta tgttgcggcc atcagagccg ctcagctcgg
      121 cttaaaaaca gccgttgtgg aaaaggaaaa actcggggga acatgtctgc ataaaggctg
      181 tatcccgagt aaagcgctgc ttagaagcgc agaggtatac cggacagctc gtgaagccga
      241 tcaattcgga gtggaaacgg ctggcgtgtc cctcaacttt gaaaaagtgc agcagcgtaa
      301 gcaagccgtt gttgataagc ttgcagcggg tgtaaatcat ttaatgaaaa aaggaaaaat
      361 tgacgtgtac accggatatg gacgtatcct tggaccgtca atcttctctc cgctgccggg
      421 aacaatttct gttgagcggg gaaatggcga agaaaatgac atgctgatcc cgaaacaagt
      481 gatcattgca acaggatcaa gaccgagaat gcttccgggt cttgaagtgg acggtaagtc
      541 tgtactgact tcagatgagg cgctccaaat ggaggagctg ccacagtcaa tcatcattgt
      601 cggcggaggg gttatcggta tcgaatgggc gtctatgctt catgattttg gcgttaaggt
      661 aacggttatt gaatacgcgg atcgcatatt gccgactgaa gatctagaga tttcaaaaga
      721 aatggaaagt cttcttaaga aaaaaggcat ccagttcata acaggggcaa aagtgctgcc
      781 tgacacaatg acaaaaacat cagacgatat cagcatacaa gcggaaaaag acggagaaac
      841 cgttacctat tctgctgaga aaatgcttgt ttccatcggc agacaggcaa atatcgaagg
      901 catcggccta gagaacaccg atattgttac tgaaaatggc atgatttcag tcaatgaaag
      961 ctgccaaacg aaggaatctc atatttatgc aatcggagac gtaatcggtg gcctgcagtt
     1021 agctcacgtt gcttcacatg agggaattat tgctgttgag cattttgcag gtctcaatcc
     1081 gcatccgctt gatccgacgc ttgtgccgaa gtgcatttac tcaagccctg aagctgccag
     1141 tgtcggctta accgaagacg aagcaaaggc gaacgggcat aatgtcaaaa tcggcaagtt
     1201 cccatttatg gcgattggaa aagcgcttgt atacggtgaa agcgacggtt ttgtcaaaat
     1261 cgtggctgac cgagatacag atgatattct cggcgttcat atgattggcc cgcatgtcac
     1321 cgacatgatt tctgaagcgg gtcttgccaa agtgctggac gcaacaccgt gggaggtcgg
     1381 gcaaacgatt cacccgcatc caacgctttc tgaagcaatt ggagaagctg cgcttgccgc
     1441 agatggcaaa gccattcatt tttaaatgag tacaaaccga catcaagcac tagggctgac
     1501 tgatcaggaa gccgttgata tgtatagaac catgctgtta gcaagaaaaa tcgatgaaag
     1561 aatgtggctg ttaaaccgtt ctggcaaaat tccatttgta atctcttgtc aaggacagga
     1621 agcagcacag gtaggagcgg ctttcgcact tgaccgtgaa atggattatg tattgccgta
     1681 ctacagagac atgggtgtcg tgctcgcgtt tggcatgaca gcaaaggact taatgatgtc
     1741 cgggtttgca aaagcagcag atccgaactc aggaggccgc cagatgccgg gacatttcgg
     1801 acaaaagaaa aaccgcattg tgacgggatc atctccggtt acaacgcaag tgccgcacgc
     1861 agtcggtatt gcgcttgcgg gacgtatgga gaaaaaggat atcgcagcct ttgttacatt
     1921 cggggaaggg tcttcaaacc aaggcgattt ccatgaaggg gcaaactttg ccgctgtcca
     1981 taagctgccg gttattttca tgtgtgaaaa caacaaatac gcaatctcag tgccttacga
     2041 taagcaagtc gcatgtgaga acatttccga ccgtgccata ggctatggga tgcctggcgt
     2101 aactgtgaat ggaaatgatc cgctggaagt ttatcaagcg gttaaagaag cacgcgaaag
     2161 ggcacgcaga ggagaaggcc cgacattaat tgaaacgatt tcttaccgcc ttacaccaca
     2221 ttccagtgat gacgatgaca gcagctacag aggccgtgaa gaagtagagg aagcgaaaaa
     2281 aagtgatccc ctgcttactt atcaagctta cttaaaggaa acaggcctgc tgtccgatga
     2341 gatagaacaa accatgctgg atgaaattat ggcaatcgta aatgaagcga cggatgaagc
     2401 ggagaacgcc ccatatgcag ctcctgagtc agcgcttgat tatgtttatg cgaagtagat
     2461 gtcagtaatg tcatatattg atgcaatcaa tttggcgatg aaagaagaaa tggaacgaga
     2521 ttctcgcgtt ttcgtccttg gggaagatgt aggaagaaaa ggcggtgtgt ttaaagcgac
     2581 agcgggactc tatgaacaat ttggggaaga gcgcgttatg gatacgccgc ttgctgaatc
     2641 tgcaatcgca ggagtcggta tcggagcggc aatgtacgga atgagaccga ttgctgaaat
     2701 gcagtttgct gatttcatta tgccggcagt caaccaaatt atttctgaag cggctaaaat
     2761 ccgctaccgc agcaacaatg actggagctg tccgattgtc gtcagagcgc catacggcgg
     2821 aggcgtgcac ggagccctgt atcattctca atcagtcgaa gcaattttcg ccaaccagcc
     2881 cggactgaaa attgtcatgc catcaacacc atatgacgcg aaagggctct taaaagccgc
     2941 agttcgtgac gaagaccccg tgctgttttt tgagcacaag cgggcatacc gtctgataaa
     3001 gggcgaggtt ccggctgatg attatgtcct gccaatcggc aaggcggacg taaaaaggga
     3061 aggcgacgac atcacagtga tcacatacgg cctgtgtgtc cacttcgcct tacaagctgc
     3121 agaacgtctc gaaaaagatg gcatttcagc gcatgtggtg gatttaagaa cagtttaccc
     3181 gcttgataaa gaagccatca tcgaagctgc gtccaaaact ggaaaggttc ttttggtcac
     3241 agaagataca aaagaaggca gcatcatgag cgaagtagcc gcaattatat ccgagcattg
     3301 tctgttcgac ttagacgcgc cgatcaaacg gcttgcaggt cctgatattc cggctatgcc
     3361 ttatgcgccg acaatggaaa aatactttat ggtcaaccct gataaagtgg aagcggcgat
     3421 gagagaatta gcggagtttt aaatggcaat tgaacaaatg acgatgccgc agcttggaga
     3481 aagcgtaaca gaggggacga tcagcaaatg gcttgtcgcc cccggtgata aagtgaacaa
     3541 atacgatccg atcgcggaag tcatgacaga taaggtaaat gcagaggttc cgtcttcttt
     3601 tactggtacg ataacagagc ttgtgggaga agaaggccaa accctgcaag tcggagaaat
     3661 gatttgcaaa attgaaacag aaggcgcgaa tccggctgaa caaaaacaag aacagccagc
     3721 agcatcagaa gccgctgaga accctgttgc aaaaagtgct ggagcagccg atcagcccaa
     3781 taaaaagcgc tactcgccag ctgttctccg tttggccgga gagcacggca ttgacctcga
     3841 tcaagtgaca ggaactggtg ccggcgggcg catcacacga aaagatattc agcgcttaat
     3901 tgaaacaggc ggcgtgcaag aacagaatcc tgaggagctg aaaacagcag ctcctgcacc
     3961 gaagtctgca tcaaaacctg agccaaaaga agagacgtca tatcctgcgt ctgcagccgg
     4021 tgataaagaa atccctgtca caggtgtaag aaaagcaatt gcttccaata tgaagcgaag
     4081 caaaacagaa attccgcatg cttggacgat gatggaagtc gacgtcacaa atatggttgc
     4141 atatcgcaac agtataaaag attcttttaa gaagacagaa ggctttaatt taacgttctt
     4201 cgcctttttt gtaaaagcgg tcgctcaggc gttaaaagaa ttcccgcaaa tgaatagcat
     4261 gtgggcgggg gacaaaatta ttcagaaaaa ggatatcaat atttcaattg cagttgccac
     4321 agaggattct ttatttgttc cggtgattaa aaacgctgat gaaaaaacaa ttaaaggcat
     4381 tgcgaaagac attaccggcc tagctaaaaa agtaagagac ggaaaactca ctgcagatga
     4441 catgcaggga ggcacgttta ccgtcaacaa cacaggttcg ttcgggtctg ttcagtcgat
     4501 gggcattatc aactaccctc aggctgcgat tcttcaagta gaatccatcg tcaaacgccc
     4561 ggttgtcatg gacaatggca tgattgctgt cagagacatg gttaatctgt gcctgtcatt
     4621 agatcacaga gtgcttgacg gtctcgtgtg cggacgattc ctcggacgag tgaaacaaat
     4681 tttagaatcg attgacgaga agacatctgt ttactaaata agcaaaaaga gcattttttg
     4741 aagttttgtt tcaaaaaatg ctctttttct atgctttat
//
