LOCUS       PWY-6466_T1  1726 bp     DNA  linear    SYN       30-JUL-2026
DEFINITION  Synthetic construct PWY-6466_T1; designed by SynPAL design pipeline.
ACCESSION   PWY-6466_T1
VERSION     PWY-6466_T1
KEYWORDS    SynPAL; synthetic construct.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /note="designed by SynPAL design pipeline"
     misc_feature    1..197
                     /note="flanking_up: upstream regulatory flank [40,800]bp"
                     /note="source organism: Bacillus subtilis subtilis 168"
     promoter        1..197
                     /note="placeholder promoter (native upstream flank)"
     gene            198..1082
                     /gene="pdxS"
                     /locus_tag="pdxS"
     CDS             198..1082
                     /gene="pdxS"
                     /locus_tag="pdxS"
                     /organism="Bacillus subtilis subtilis 168"
                     /note="native genomic strand +"
     gene            1083..1673
                     /gene="pdxT"
                     /locus_tag="pdxT"
     CDS             1083..1673
                     /gene="pdxT"
                     /locus_tag="pdxT"
                     /organism="Bacillus subtilis subtilis 168"
                     /note="native genomic strand +"
     misc_feature    1674..1726
                     /note="flanking_down: downstream regulatory flank [40,800]bp"
                     /note="source organism: Bacillus subtilis subtilis 168"
     terminator      1674..1726
                     /note="placeholder terminator (native downstream flank)"
ORIGIN
        1 atcaattgaa agagctctga tggatgttag ggctctttcg cgtaatatac tgaaaaaacc
       61 caatttttaa taaaaaatga ttttcaaagc gctaaaaacc gaataaaata gtatatatgc
      121 atatgaatta ttggatttct aggatacaat aaggattaga aatcatataa ctataccttg
      181 attaggggga ccaagaaatg gctcaaacag gtactgaacg tgtaaaacgc ggaatggcag
      241 aaatgcaaaa aggcggcgtc atcatggacg tcatcaatgc ggaacaagcg aaaatcgctg
      301 aagaagctgg agctgtcgct gtaatggcgc tagaacgtgt gccagcagat attcgcgcgg
      361 ctggaggagt tgcccgtatg gctgacccta caatcgtgga agaagtaatg aatgcagtat
      421 ctatcccggt aatggcaaaa gcgcgtatcg gacatattgt tgaagcgcgt gtgcttgaag
      481 ctatgggtgt tgactatatt gatgaaagtg aagttctgac gccggctgac gaagaatttc
      541 atttaaataa aaatgaatac acagttcctt ttgtctgtgg ctgccgtgat cttggtgaag
      601 caacacgccg tattgcggaa ggtgcttcta tgcttcgcac aaaaggtgag cctggaacag
      661 gtaatattgt tgaggctgtt cgccatatgc gtaaagttaa cgctcaagtg cgcaaagtag
      721 ttgcgatgag tgaggatgag ctaatgacag aagcgaaaaa cctaggtgct ccttacgagc
      781 ttcttcttca aattaaaaaa gacggcaagc ttcctgtcgt taactttgcc gctggcggcg
      841 tagcaactcc agctgatgct gctctcatga tgcagcttgg tgctgacgga gtatttgttg
      901 gttctggtat ttttaaatca gacaaccctg ctaaatttgc gaaagcaatt gtggaagcaa
      961 caactcactt tactgattac aaattaatcg ctgagttgtc aaaagagctt ggtactgcaa
     1021 tgaaagggat tgaaatctca aacttacttc cagaacagcg tatgcaagaa cgcggctggt
     1081 aaatgttaac aataggtgta ctaggacttc aaggagcagt tagagagcac atccatgcga
     1141 ttgaagcatg cggcgcggct ggtcttgtcg taaaacgtcc ggagcagctg aacgaagttg
     1201 acgggttgat tttgccgggc ggtgagagca cgacgatgcg ccgtttgatc gatacgtatc
     1261 aattcatgga gccgcttcgt gaattcgctg ctcagggcaa accgatgttt ggaacatgtg
     1321 ccggattaat tatattagca aaagaaattg ccggttcaga taatcctcat ttaggtcttc
     1381 tgaatgtggt tgtagaacgt aattcatttg gccggcaggt tgacagcttt gaagctgatt
     1441 taacaattaa aggcttggac gagcctttta ctggggtatt catccgtgct ccgcatattt
     1501 tagaagctgg tgaaaatgtt gaagttctat cggagcataa tggtcgtatt gtagccgcga
     1561 aacaggggca attccttggc tgctcattcc atccggagct gacagaagat caccgagtga
     1621 cgcagctgtt tgttgaaatg gttgaggaat ataagcaaaa ggcacttgta taaaacagtt
     1681 gaaagctgtg gaaacttata gtacattata agcacaaata aagatc
//
