LOCUS       PWY-66_T1    3184 bp     DNA  linear    SYN       30-JUL-2026
DEFINITION  Synthetic construct PWY-66_T1; designed by SynPAL design pipeline.
ACCESSION   PWY-66_T1
VERSION     PWY-66_T1
KEYWORDS    SynPAL; synthetic construct.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /note="designed by SynPAL design pipeline"
     misc_feature    1..317
                     /note="flanking_up: upstream regulatory flank [40,800]bp"
                     /note="source organism: Agrobacterium fabrum C58"
     promoter        1..317
                     /note="placeholder promoter (native upstream flank)"
     gene            318..1388
                     /gene="gmd"
                     /locus_tag="gmd"
     CDS             318..1388
                     /gene="gmd"
                     /locus_tag="gmd"
                     /organism="Agrobacterium fabrum C58"
                     /note="native genomic strand +"
     gene            1389..2384
                     /gene="fcl"
                     /locus_tag="fcl"
     CDS             1389..2384
                     /gene="fcl"
                     /locus_tag="fcl"
                     /organism="Agrobacterium fabrum C58"
                     /note="native genomic strand +"
     misc_feature    2385..3184
                     /note="flanking_down: downstream regulatory flank [40,800]bp"
                     /note="source organism: Agrobacterium fabrum C58"
     terminator      2385..3184
                     /note="placeholder terminator (native downstream flank)"
ORIGIN
        1 tccaatccgt ctccatgaat cgtagatcat gtcgtctcgc gctccctgct accttaagag
       61 atagtgcgcg acccggcgtt gtatttccga agtctggcac gacctgcggg acctgcaact
      121 cgaccaaaag gcttaagtcg aaagcggcaa tactcaccct gcctcctcta aaaggcagat
      181 gtcgacgtgg tggacggtga atcggccagt tctgacttgc cgtcctctcc cttttggtat
      241 gctgccgatg cgcagggcct cgttatacct ttcagggagg cgccatccgg ggccggaacc
      301 gctgggagat gtggtggatg gtagacaaga gagccttgat tatcggcgta accgggcagg
      361 atggcgctta tctcagcgaa ttgctgcttg ccaagggata tatggttcac ggcctgaaga
      421 ggcggtcttc ctcctttaat accggtcgga tcgatcatct ttaccaagat cccttcgagg
      481 aagacctgcg ctttcgcctg cacttcggtg acctcacgga cgccacgaac ctgtgccggg
      541 tcattcagga ggtccagccc gacgagatct acaatctagg cgcccagagc catgtccagg
      601 tcagcttcga gacgccggaa tatacggcaa atgcggatgc gcttggcacg ctgaggctgc
      661 tggaagccat gcgtatcctg aaccttggcg atcgctgccg gttttaccag gcatccacat
      721 cggagctgtt cggggggatg ggcacggtcg cccagcacga gggtacgccg ttctatccga
      781 gaagccccta tgctgcggcc aaactctacg catactggat gacggtcaat taccgggaaa
      841 gttacggctt tcacgcatcc aacggcattc tcttcaacca tgagagcccc attcgcgggg
      901 agacattcgt cacccggaag atcacgcgtg gcgttgcggc catcgagcgc ggtctcatga
      961 gcaagctgcg tcttggcaat ctcgacgcaa agagggactg ggggcatgcg cgcgattatg
     1021 tcgaaggcat gtggcgcatc gtgcagcagg aaatacccga ggactatgtg cttgcgaccg
     1081 gcgaagcaca tacggtgcgg gaatttgttg agcttgcttt cggcgtggtt ggcaagacca
     1141 tcgaatggca cggcgatggc agtgacgaag tgggatatga tgccaaaacc ggcaattcga
     1201 tcatcgaaat cgatcccaga tattttcgtc cggcggaagt ggattttctt ctcggcgatc
     1261 ccagcaaggc gcgcagaaaa ctcgactgga gtcactcgac gtccttttgc gacctcgttc
     1321 aggaaatggt cgaagcggat cttcgcctcg taatgttgga agagggaaga aatgagttcg
     1381 tcgcctgaat gagttcgtcg cctgaatcga aagacatgcc gttcagtcta aacggcaagc
     1441 gtgtctgggt tgccggccat acgggtatgg tcggctcggc actcgtgcga cgtctggagc
     1501 gcgagaattg cgaaatcctc aaagtctccc gccgcgagct tgatctgacc cgacaatacg
     1561 agacggagca atggatggcg gcggcaaggc cgcaggtcat ttttgtcgct gccgccaagg
     1621 tcggaggcat cgcagcaaat gcagcctatc cggcggactt tctttacacc aacacgctca
     1681 tttcgatgaa tatcatgaaa tcggctgccg atatcggtgt cgaaaagctt ctctggatgg
     1741 gatctagctg catctatccg aaattcgcag ctcagccgat cacggaaaat gcccttttga
     1801 caggtccgct ggagcctacc aacgaggcct acgcgattgc gaagatcgcg gcgctcaagc
     1861 tttcccaatt ctattccatt cagtacggcc tgaattgcgt ctccgtcatg ccgaccaata
     1921 tttacgggct gaacgacaat ttcgatccgc aaagctcgca tgtcattccc gcgatgatca
     1981 ggagaatgca tgaggcgaaa atcagcggcc agaacaagat cgttctatgg ggtacgggca
     2041 gtcctctgcg tgaatttctc catgtggacg atctggccga tgcctgttgt ttcctcatga
     2101 agtcatctgc gcattttccg ctgatcaata tcggctcggg gcgggagata tcaatccgca
     2161 atctcgccca tctgattgca ggcatcgtcg gttacgaggg gcagatcgtt tttgacacca
     2221 gcaaaccgga tggagccccc agaaagctgc tcgactgctc gcggctcaac gcgcttggct
     2281 ggaactcgac ggtcgaactc agatacggaa ttcaggattt gtatgagtgg tggcgacacc
     2341 cgaagtcgct gcaatcggat atgttacgac aagcggcaag atgagatcgc caaattccct
     2401 aatatcatca attttgcttg ccgcccttgc gctcgcattt tcaggtctct cgtttgccgg
     2461 gaagcccgcg gcgcagggag tgcaagagca aggcagccaa cccgatccac ggcagttcgg
     2521 tttcccgata cctgtcccgg aaccacgatt gcctgacgat gccgatgcct tcaatccgcc
     2581 gtggatggag gtcggcggca aggggccggc aatcgcggag atttcgagaa cggcgggcgc
     2641 tggcgaggtt gtctcgatga ccggcgtcga tctcgacgga cgaacgggtt tcgaagtctt
     2701 ttcgcaattc cccggtgccg cgcagggcgt cgtgacgccg ttggcaccgc tgacggctga
     2761 cgcaacggcg gcgacgctgc tgctgcctgc cacgcttcct tcatggtcca cctacctgat
     2821 caggcctctg cggggcgatc tcgaagggcg cagctttgcg atcaatcgca cggaaagctg
     2881 gtggatcggg cccgacgccg cagcgccggg agcgatcgtc tcggtctatg gccgcaacct
     2941 ttcgcggttc aacggcacca gccaatccta tctctatgtc aagccggccg gtgccgccgg
     3001 ctggtacgtc aaggcaatct ccgtcaatcc cttcaaggtc gatttcaagg tgctggctct
     3061 gccgccggcg cgttatgaag tatgggttca caatactcat ggcggccgtt tcggctggag
     3121 cggtccgctg acacttgatg tcctgtcgca atcgccatgg gcggcgcagg ataaaaagcg
     3181 catc
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