LOCUS       PWY0-1324_T1 2506 bp     DNA  linear    SYN       30-JUL-2026
DEFINITION  Synthetic construct PWY0-1324_T1; designed by SynPAL design pipeline.
ACCESSION   PWY0-1324_T1
VERSION     PWY0-1324_T1
KEYWORDS    SynPAL; synthetic construct.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /note="designed by SynPAL design pipeline"
     misc_feature    1..122
                     /note="flanking_up: upstream regulatory flank [40,800]bp"
                     /note="source organism: Escherichia coli K-12 substr. MG1655"
     promoter        1..122
                     /note="placeholder promoter (native upstream flank)"
     gene            123..1016
                     /gene="nanA"
                     /locus_tag="nanA"
     CDS             123..1016
                     /gene="nanA"
                     /locus_tag="nanA"
                     /organism="Escherichia coli K-12 substr. MG1655"
                     /note="native genomic strand -"
     gene            1017..1706
                     /gene="nanE"
                     /locus_tag="nanE"
     CDS             1017..1706
                     /gene="nanE"
                     /locus_tag="nanE"
                     /organism="Escherichia coli K-12 substr. MG1655"
                     /note="native genomic strand -"
     misc_feature    1707..2506
                     /note="flanking_down: downstream regulatory flank [40,800]bp"
                     /note="source organism: Escherichia coli K-12 substr. MG1655"
     terminator      1707..2506
                     /note="placeholder terminator (native downstream flank)"
ORIGIN
        1 atgccacttt agtgaagcag atcgcattat aagctttctg tatggggtgt tgcttaattg
       61 atctggtata acaggtataa aggtatatcg tttatcagac aagcatcact tcagaggtat
      121 ttatggcaac gaatttacgt ggcgtaatgg ctgcactcct gactcctttt gaccaacaac
      181 aagcactgga taaagcgagt ctgcgtcgcc tggttcagtt caatattcag cagggcatcg
      241 acggtttata cgtgggtggt tcgaccggcg aggcctttgt acaaagcctt tccgagcgtg
      301 aacaggtact ggaaatcgtc gccgaagagg cgaaaggtaa gattaaactc atcgcccacg
      361 tcggttgcgt cagcaccgcc gaaagccaac aacttgcggc atcggctaaa cgttatggct
      421 tcgatgccgt ctccgccgtc acgccgttct actatccttt cagctttgaa gaacactgcg
      481 atcactatcg ggcaattatt gattcggcgg atggtttgcc gatggtggtg tacaacattc
      541 cagccctgag tggggtaaaa ctgaccctgg atcagatcaa cacacttgtt acattgcctg
      601 gcgtaggtgc gctgaaacag acctctggcg atctctatca gatggagcag atccgtcgtg
      661 aacatcctga tcttgtgctc tataacggtt acgacgaaat cttcgcctct ggtctgctgg
      721 cgggcgctga tggtggtatc ggcagtacct acaacatcat gggctggcgc tatcagggga
      781 tcgttaaggc gctgaaagaa ggcgatatcc agaccgcgca gaaactgcaa actgaatgca
      841 ataaagtcat tgatttactg atcaaaacgg gcgtattccg cggcctgaaa actgtcctcc
      901 attatatgga tgtcgtttct gtgccgctgt gccgcaaacc gtttggaccg gtagatgaaa
      961 aatatctgcc agaactgaag gcgctggccc agcagttgat gcaagagcgc gggtgaatgt
     1021 cgttacttgc acaactggat caaaaaatcg ctgctaacgg tggcctgatt gtctcctgcc
     1081 agccggttcc ggacagcccg ctcgataaac ccgaaatcgt cgccgccatg gcattagcgg
     1141 cagaacaggc gggcgcggtt gccattcgca ttgaaggtgt ggcaaatctg caagccacgc
     1201 gtgcggtggt gagcgtgccg attattggaa ttgtgaaacg cgatctggag gattctccgg
     1261 tacgcatcac ggcctatatt gaagatgttg atgcgctggc gcaggcgggc gcggacatta
     1321 tcgccattga cggcaccgac cgcccgcgtc cggtgcctgt tgaaacgctg ctggcacgta
     1381 ttcaccatca cggtttactg gcgatgaccg actgctcaac gccggaagac ggcctggcat
     1441 gccaaaagct gggagccgaa attattggca ctacgctttc tggctatacc acgcctgaaa
     1501 cgccagaaga gccggatctg gcgctggtga aaacgttgag cgacgccgga tgtcgggtga
     1561 ttgccgaagg gcgttacaac acgcctgctc aggcggcgga tgcgatgcgc cacggcgcgt
     1621 gggcggtgac ggtcggttct gcaatcacgc gtcttgagca catttgtcag tggtacaaca
     1681 cagcgatgaa aaaggcggtg ctatgaccac actggcgatt gatatcggcg gtactaaact
     1741 tgccgccgcg ctgattggcg ctgacgggca gatccgcgat cgtcgtgaac ttcctacgcc
     1801 agccagccag acaccagaag ccttgcgtga tgccttatcc gcattagtct ctccgttgca
     1861 agctcatgcg cagcgggttg ccatcgcttc gaccgggata atccgtgacg gcagcttgct
     1921 ggcgcttaat ccgcataatc ttggtggatt gctacacttt ccgttagtca aaacgctgga
     1981 acaacttacc aatttgccga ccattgccat taacgacgcg caggccgcag catgggcgga
     2041 gtttcaggcg ctggatggcg atataaccga tatggtcttt atcaccgttt ccaccggcgt
     2101 tggcggcggt gtagtgagcg gctgcaaact gcttaccggc cctggcggtc tggcggggca
     2161 tatcgggcat acgcttgccg atccacacgg cccagtctgc ggctgtggac gcacaggttg
     2221 cgtggaagcg attgcttctg gtcgcggcat tgcagcggca gcgcaggggg agttggctgg
     2281 cgcggatgcg aaaactattt tcacgcgcgc cgggcagggt gacgagcagg cgcagcagct
     2341 gattcaccgc tccgcacgta cgcttgcaag gctgatcgct gatattaaag ccacaactga
     2401 ttgccagtgc gtggtggtcg gtggcagcgt tggtctggca gaagggtatc tggcgctggt
     2461 ggaaacgtat ctggcgcagg agccagcggc atttcatgtt gattta
//
