N-acetylneuraminate and N-acetylmannosamine degradation I
PWY0-1324Recommended ordernanAnanE
Source
- Escherichia coli K-12 substr. MG1655chosen
Design gallery
Showcase design constructs
Each construct is the top-scoring arrangement of its pathway, extracted with native regulatory flanks and ranked by design-readiness tier. All files are static downloads — no accounts or API keys.
- Constructs
- 6
- T2 candidates screened
- 13
- T3 candidates screened
- 7
Single co-directional operon in one organism; reconstructable as one cassette.
T1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA
PWY-5046Recommended orderlpdVbkdAAbkdABbkdB
Source
- Bacillus subtilis subtilis 168chosen
pyridoxal 5'-phosphate biosynthesis II
PWY-6466Recommended orderpdxSpdxT
Source
- Bacillus subtilis subtilis 168chosen
GDP-L-fucose biosynthesis I (from GDP-D-mannose)
PWY-66Recommended ordergmdfcl
Source
- Agrobacterium fabrum C58chosen
Multi-module, same species; several co-directional cassettes from one host.
NADH to trimethylamine N-oxide electron transfer
PWY0-1347Recommended ordernuoAnuoBnuoCnuoEnuoFnuoGnuoHnuoInuoJnuoKnuoLnuoMnuoNtorAtorC
Source
- Salmonella enterica enterica serovar Typhimurium str. LT2chosen
Multi-species assembly; modules combined across organisms.
glycine cleavage
GLYCLEAV-PWYRecommended ordergcvTgcvHgcvPlpd
Sources
- Escherichia coli K-12 substr. MG1655
- Sinorhizobium meliloti 1021chosen
primer_method: wallace_heuristic_v1
Primers use wallace_heuristic_v1 (Wallace rule Tm on short binding regions with placeholder vector-homology tails). Not Primer3; re-design before ordering oligos. Primers use the Wallace ruleTm = 2·(A+T) + 4·(G+C) on short binding regions with placeholder vector-homology tails — not Primer3. Re-design before ordering oligos.
Full method: design pipeline · per-pathway evidence: the atlas.